| Protocol for mapping Tcell activation using single-cell RNA-seq |
| Author |
Li, Hui; Liu, Yifei; Wang, Xuefei; Yu, Shiya; Wang, Junliang; Hu, Yue; Hong, Ni; Jin, Wenfei |
| Journal |
STAR protocols |
| Pub Year |
2024 |
| Type |
|
| Abstract |
Stimulation of CD4 Tcells with anti-CD3/CD28 is a commonly used model to study Tcell activation. Here, we present a protocol for investigating Tcell activation based on anti-CD3/CD28 bead stimulation and single-cell RNA sequencing (scRNA-seq). We describe the workflow from the isolation of human peripheral blood mononuclear cells (PMBCs) and CD4 Tcell enrichment to anti-CD3/CD28 bead stimulation, scRNA-seq, and data analysis. For complete details on the use and execution of this protocol, please refer to Li etal.1. |
| Issue |
5 |
| Volume |
5 |
| SCI |
1.3 |
