| Mapping of clonal lineages across developmental in human neural differentiation |
| Author |
You, ZW; Wang, LY; He, H; Wu, ZY; Zhang, XY; Xue, SX; Xu, PB; Hong, YH; Xiong, M; Wei, W; Chen, YJ |
| Journal |
CELL STEM CELL |
| Pub Year |
2023 |
| Type |
Article |
| Abstract |
The cell lineages across developmental stages remain to be elucidated. Here, we developed single-cell split barcoding (SISBAR) that allows clonal tracking of single-cell transcriptomes across stages in an in vitro model of human ventral midbrain-hindbrain differentiation. We developed potential-spectiveand origin-spectiveanalyses to investigate the cross-stage lineage relationships and mapped a multi-level clonal lineage landscape depicting the whole differentiation process. We uncovered many previously unchar-acterized converging and diverging trajectories. Furthermore, we demonstrate that a transcriptome-defined cell type can arise from distinct lineages that leave molecular imprints on their progenies, and the multilineage fates of a progenitor cell-type represent the collective results of distinct rather than similar clonal fates of individual progenitors, each with distinct molecular signatures. Specifically, we uncovered a ventral midbrain progenitor cluster as the common clonal origin of midbrain dopaminergic (mDA) neurons, midbrain glutama-tergic neurons, and vascular and leptomeningeal cells and identified a surface marker that can improve graft outcomes. |
| Issue |
30 |
| Volume |
30 |
| SCI |
23.9 |
