| MATR3-antisense LINE1 RNA meshwork scaffolds higher-order chromatin organization |
| Author |
Zhang, YW; Cao, X; Gao, ZH; Ma, XY; Wang, QF; Xu, XX; Cai, XM; Zhang, Y; Zhang, Z; Wei, G; Wen, B |
| Journal |
EMBO REPORTS |
| Pub Year |
2023 |
| Type |
Article |
| Abstract |
Long interspersed nuclear elements (LINEs) play essential roles in shaping chromatin states, while the factors that cooperate with LINEs and their roles in higher-order chromatin organization remain poorly understood. Here, we show that MATR3, a nuclear matrix protein, interplays with antisense LINE1 (AS L1) RNAs to form a meshwork via phase separation, providing a dynamic platform for chromatin spatial organization. MATR3 and AS L1 RNAs affect the nuclear localization of each other. After MATR3 depletion, the chromatin, particularly H3K27me3-modified chromatin, redistributes in the cell nuclei. Topologically associating domains (TADs) that highly transcribe MATR3-associated AS L1 RNAs show decreased intra-TAD interactions in both AML12 and ES cells. MATR3 depletion increases the accessibility of H3K27me3 domains adjacent to MATR3-associated AS L1, without affecting H3K27me3 modifications. Furthermore, amyotrophic lateral sclerosis (ALS)-associated MATR3 mutants alter biophysical features of the MATR3-AS L1 RNA meshwork and cause an abnormal H3K27me3 staining. Collectively, we reveal a role of the meshwork formed by MATR3 and AS L1 RNAs in gathering chromatin in the nucleus. |
| Issue |
24 |
| Volume |
24 |
| SCI |
7.7 |
