Research Areas:

1. Using human pluripotent stem cells to model, study and treat diseases

2. The genetic and epigenetic regulation of liver lipid metabolism

3. Novel genetic therapeutics to metabolic diseases

Brief Biography:

Education/Research Experience:

2017~present Principal Investigator, Shanghai Institute of Nutrition and Health (SINH), Chinese Academy of Sciences (CAS)

2014.09~2016 Principal Investigator, Institute for Nutritional Sciences (INS), Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS)

2010.09~2014.08 Postdoctoral Fellow, Harvard Stem Cell Institute, Harvard Department of Stem Cell and Regenerative Biology

2004.09~2010.03 Ph.D. Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS)

2000.09~2004.07 B.S. Nanjing University

Academic Awards:

2015 Shanghai Pujang Talent Award

2014 ATVB Young Investigator Award for Women (Finalist)

2014 American Heart Association Fellowship

2010 Roche Fellowship

Selected Publications: (*Corresponding Author)

1. Zhao Y^#*, Li S^#, Chen Y, Wang Y, Wei Y, Zhou T, Zhang Y, Yang Y, Chen L, Liu Y, Hu C, Zhou B, Ding Q*. Histone phosphorylation integrates the hepatic glucagon-PKA-CREB gluconeogenesis program in response to fasting. Mol Cell 2023 Apr 6;83(7):1093-1108
2. Chen Y^#*, Chen L^#, Wu X^#, Zhao Y, Wang Y, Jiang D, Liu X, Zhou T, Li S, Wei Y, Liu Y, Hu C, Zhou B, Qin J, Ying H, Ding Q*. Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice. Nat Commun 2023 Mar 18;14(1):1521
3. Wu X, Jiang D, Li S*, Ding Q*. Modeling drug-induced liver injury and screening for anti-hepatofibrotic compounds using human PSC-derived organoids. Cell Regen 2023 Mar 3;12(1):6
4. Tian C, Min X, Zhao Y, Wang Y, Wu X, Liu S, Dou W, Zhou T, Liu Y, Luo R, Li Z, Lui KO, Li Y, Zhou B, Ding Q*. MRG15 aggravates non-alcoholic steaohepatitis progression by regulating the mitochondrial proteolytic degradation of TUFM. J Hepatol 2022 Dec;77(6):1491-1503
5. Zhou T, Musunuru K, Lui K*, Ding Q*. Decoding liver fibrogenesis with single-cell technologies. Life Med 2022 Dec;1(3):333-344
6. Han J^#*, Wang Y^#*, Qiu Y, Sun D, Liu Y, Li Z, Zhou B, Zhang H, Xiao Y, Wu G*, Ding Q*. Single-cell sequencing unveils key contributions of immune cell populations in cancer-associated adipose wasting. Cell Disc 2022 Nov 15;8(1):122
7. Chen Y, Ding Q*. Optimized protocols for efficient gene editing in mouse hepatocytes in vivo using CRISPR-Cas9 technology. STAR Protoc 2021 Dec 23;3(1):101062
8. Qiu Y, Liu X, Sun Y, Zeng X, Li S, Wei Y, Tian C, Ding Q*. In situ saturating mutagenesis screening identifies a functional genomic locus that regulates Ucp1 expression. Phenomics 2021 Feb 22;1(1):15-21
9. Qiu Y, Ding Q*. Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology. STAR Protoc 2021 Jan 27;2(1):100307
10. Liu X, Yang Y, Qiu Y, Md Reyad-Ul-Ferdous, Ding Q*, Wang Y*. SeqCor: correct the effect of gRNA sequences in CRISPR/Cas9 screenings by machine learning algorithm. J Genet Genomics 2020 Nov 20;47(11):672-680
11. Qiu Y, Yang Y, Wei Y, Liu X, Feng Z, Zeng X, Chen Y, Liu Y, Zhao Y, Chen L, Luo L, Ding Q*. Glyburide regulates UCP1 expression in adipocytes independent of K ATP channel blockade. iScience 2020 Aug 10;23(9):101446
12. Ding Q*. Spotlight on gene therapy in China. Gene Ther 2020 Aug;27(7-8):307-308
13. Wei Y^#, Tian C^#, Zhao Y^#, Liu X, Liu F, Li S, Chen Y, Qiu Y, Feng Z, Chen L, Zhou T, Ren X, Feng C, Liu Y, Yu W, Ying H, Ding Q*. MRG15 orchestrates rhythmic epigenomic remodeling and controls hepatic metabolism. Nat Metab 2020 May;2(5):447-460
14. Yang Y^#, Liu X^#, Li S, Chen Y, Zhao Y, Wei Y, Qiu Y, Liu Y, Zhou Z, Han J*, Wu G*, Ding Q*. Genome-scale CRISPR screening for potential targets of ginsenoside compound K. Cell Death Dis 2020 Jan 20;11(1):39
15. Zhao Y^#, Feng Z^#, Ding Q*. Type 2 Diabetes Variants in the SLC16A11 coding region are not loss-of-function mutations. Cell Rep 2019 Oct 15;29(3):781-784
16. Li S, Huang S, Zhao Y, Ding Y, Ma D, Ding Q*. Derivation and applications of human hepatocyte-like cells. World J Stem Cells 2019 Aug 26;11(8):535-547
17. Feng Z, Wei Y, Zhang Y, Qiu Y, Liu X, Su L, Liang N, Yin H, Ding Q*. Identification of a rhodanine derivative BML-260 as a potent stimulator of UCP1 expression. Theranostics 2019 May 26;9(12):3501-3514
18. Zhao Y^#, Feng Z^#, Zhang Y^#, Sun Y, Chen Y, Liu X, Li S, Zhou T, Chen L, Wei Y, Ma D, Lui K, Ying H, Chen Y, Ding Q*. Gain-of-function mutations of SLC16A11 contribute to the pathogenesis of type 2 diabetes. Cell Rep 2019 Jan 22;26(4):884-892
19. Qiu Y^#, Sun Y^#, Xu D, Yang Y, Liu X, Wei Y, Chen Y, Feng Z, Li S, Ferdous M, Zhao Y, Xu H, Lao Y*, Ding Q*. Screening of FDA-approved drugs identifies sutent as a modulator of UCP1 expression in brown adipose tissue. EBioMedicine 2018 Nov;37:344-355
20. Li S, Li M, Liu X, Yang Y, Wei Y, Chen Y, Qiu Y, Zhou T, Feng Z, Ma D, Fang J, Ying H, Wang H, Musunuru K, Shao S*, Zhao Y*, Ding Q*. Genetic and chemical screenings identify HDAC3 as a key regulator in hepatic differentiation of human pluripotent stem cells. Stem Cell Rep 2018 Jul 10;11(1):22-31
21. Sun Y, Ding Q*. Genome engineering of stem cell organoids for disease modeling. Protein Cell 2017 May;8(5):315-327
22. Wei Y, Qiu Y, Chen Y, Liu G, Zhang Y, Xu L, Ding Q*. CRISPR/Cas9 with single guide RNA expression driven by small tRNA promoters showed reduced editing efficiency compared to U6 promoter. RNA 2017 Jan;23(1):1-5
23. Wei Y, Chen Y, Qiu Y, Zhao H, Liu G, Zhang Y, Meng Q, Wu G, Chen Y, Cai X, Wang H, Ying H, Zhou B, Liu M, Li D, Ding Q*. Prevention of muscle wasting by CRISPR/Cas9-mediated disruption of myostatin in vivo. Mol Ther 2016 Nov;24(11):1889-1891
24. Chen Y, Liu X, Zhang Y, Wang H, Ying H, Liu M, Li D, Lui K, Ding Q*. A Self-restricted CRISPR System to Reduce Off-target Effects. Mol Ther 2016 Sep;24(9):1508-1510
25. Wang X, Raghavan A, Chen T, Qiao L, Zhang Y, Ding Q*, Musunuru K*. CRISPR-Cas9 Targeting of PCSK9 in Human Hepatocytes In Vivo-Brief Report. Arterioscler Thromb Vasc Biol 2016 May;36(5):783-786
26. Ding Q, Strong A, Patel KM, Ng SL, Gosis BS, Regan SN, Rader DJ, Musunuru K. Permanent alteration of PCSK9 with in vivo CRISPR-Cas9 genome editing. Circ Res 2014 Aug 15;115(5):488-492
27. Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K. Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs. Cell Stem Cell 2013 Apr 4;12(4):393-394
28. Ding Q^#, Lee YK^#, Schaefer EA^#, Peters DT, Veres A, Kim K, Kuperwasser N, Motola DL, Meissner TB, Hendriks WT, Trevisan M, Gupta RM, Moisan A, Banks E, Friesen M, Schinzel RT, Xia F, Tang A, Xia Y, Figueroa E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin LL, Peng LF, Chung RT, Musunuru K, Cowan CA. A TALEN genome-editing system for generating human stem cell-based disease models. Cell Stem Cell 2013 Feb 7;12(2):238-251 (^#equal contribution)
29. Ding Q, Cowan CA. Liver in a dish. Cell Res 2013 Nov;23(11):1242-1243
30. Jin T^#, Ding Q^#, Huang H, Xu D, Jiang Y, Zhou B, Li Z, Jiang X, He J, Liu W, Zhang Y, Pan Y, Wang Z, Thomas WG, Chen Y. PAQR10 and PAQR11 mediate Ras signaling in the Golgi apparatus. Cell Res 2012 Apr;22(4):661-676 (^#equal contribution)
31. Luo X^#, Ding Q^#, Wang M^#, Li Z, Mao K, Sun B, Zang YQ, Chen Y. In vivo disruption of TGF-β signaling by Smad7 in airway epithelium alleviates allergic asthma but aggravates lung carcinogenesis. PLoS ONE 2010 Apr 13;5(4):e10149 (^#equal contribution)
32. Ding Q, Wang Z, Chen Y. Endocytosis of adiponectin receptor 1 through a clathrin- and Rab5-dependent pathway. Cell Res 2009 Mar;19(3):317-327
33. Ding Q, Jin T, Wang Z, Chen Y. Catalase potentiates retinoic acid-induced THP-1 monocyte differentiation into macrophage through inhibition of peroxisome proliferator-activated receptor gamma. J Leukoc Biol 2007 Jun;81(6):1568-1576